GERMINATION • successive constraints on the process
- 01ImbibitionWater restores hydration to seed structures
- 02RespirationOxygen and reserves support metabolic changes in the embryo
- 03Growth activationTemperature and light modify the response
- 04Root emergenceThe embryonic root penetrates the seed coat
- 05Seedling establishmentLight, roots and gradual hardening sustain growth
A factor outside the tolerance range halts the process. Moisture alone cannot replace oxygen, a suitable temperature or a living embryo. [Barrios et al. 2020b] [Fleischer and Schütz 1978]
Sequence and conditions
- Record the current name, the name on the original label, field number, seed source, harvest and storage conditions. Do not combine all batches of the same species. [Flores Martínez et al. 2002, file p. 4, 5, 9, 10] [Flores Martínez and Manzanero Medina 2003, file p. 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13] [Rodríguez Ortega and Franco 2001, file p. 2, 3, 4, 5] [Kaktusy i Inne 2005b, file p. 44]
- Retain an untreated control when comparing soaking, heat, cold or scarification. Record the number of seeds and replicates. [Flores Martínez et al. 2002, file p. 4, 5, 9, 10] [Flores Martínez and Manzanero Medina 2003, file p. 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13] [Rodríguez Ortega and Franco 2001, file p. 2, 3, 4, 5] [Kaktusy i Inne 2005b, file p. 44]
- Distinguish germination, marked by radicle emergence, from seedling emergence and the number of living seedlings after pricking out. High germination does not mean successful plant production. [Flores Martínez et al. 2002, file p. 4, 5, 9, 10] [Flores Martínez and Manzanero Medina 2003, file p. 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13] [Rodríguez Ortega and Franco 2001, file p. 2, 3, 4, 5] [Kaktusy i Inne 2005b, file p. 44]
- Record day/night and substrate temperatures, lighting, humidity, watering and time to the event separately. [Flores Martínez et al. 2002, file p. 4, 5, 9, 10] [Flores Martínez and Manzanero Medina 2003, file p. 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13] [Rodríguez Ortega and Franco 2001, file p. 2, 3, 4, 5] [Kaktusy i Inne 2005b, file p. 44]
- When seeds are retained in the stem, do not automatically cut open a healthy plant. Materials on retention describe a biological mechanism, not a reliable harvesting technique. [Flores Martínez et al. 2002, file p. 4, 5, 9, 10] [Flores Martínez and Manzanero Medina 2003, file p. 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13] [Rodríguez Ortega and Franco 2001, file p. 2, 3, 4, 5] [Kaktusy i Inne 2005b, file p. 44]
- Vegetative propagation increases the number of specimens, but not the number of genotypes. Two offsets of the same plant may not suffice for outcrossed seed reproduction. [Flores Martínez et al. 2002, file p. 4, 5, 9, 10] [Flores Martínez and Manzanero Medina 2003, file p. 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13] [Rodríguez Ortega and Franco 2001, file p. 2, 3, 4, 5] [Kaktusy i Inne 2005b, file p. 44]
Scope of the method
Retention in the mother plant is not equivalent to storing dry seeds in a room.
Sources for the description
[Flores Martínez et al. 2002, file p. 4, 5, 9, 10] [Flores Martínez and Manzanero Medina 2003, file p. 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13] [Rodríguez Ortega and Franco 2001, file p. 2, 3, 4, 5] [Kaktusy i Inne 2005b, file p. 44]