CACTIBOTANY AND CULTIVATION
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Research into cactus biology

Publications from the current year. Research questions, methods and findings in short summaries with sources.

Publication year 2026 · 10 summaries per page

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Biology and physiology

Heat and limited water affect cactus germination in different ways

Cactus seeds differ in their ability to begin growing at higher temperatures and with limited water availability. A 2026 study compared five species from the southern Chihuahuan Desert, Echinocactus platyacanthus, Ferocactus pilosus, Mammillaria bocasana, Myrtillocactus geometrizans and Stenocereus huastecorum. Germination was monitored for 30 days at 25 or 32°C, with water availability controlled using polyethylene glycol solutions at water potentials of 0, −0.2 or −0.4 MPa. In Echinocactus platyacanthus and Ferocactus pilosus, the proportion of germinating seeds declined both at the higher temperature and at lower water potentials. Myrtillocactus geometrizans maintained high germination across the tested conditions, while Stenocereus huastecorum germinated better at 32°C than at 25°C. Germination of Mammillaria bocasana was constrained primarily by the higher temperature. [González-Salvatierra et al. 2026]

Significance of the study

The study identifies conditions for the onset of seed growth in individual species within a controlled experiment. Its findings can inform temperature and water availability during propagation and analyses of population recruitment under a changing climate. [González-Salvatierra et al. 2026]

Source publication

González-Salvatierra C., Zárate-Camargo G., Cach-Pérez M.J., Flores J., Sensitivity of Chihuahuan Desert cacti to heat and water limitation during germination, Flora, 2026, 341:153012, doi: 10.1016/j.flora.2026.153012.

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Biotechnology and applications

Kroenleinia grusonii from new shoots in vitro to plants in substrate

The shoot tips of young Kroenleinia grusonii seedlings were used to develop a method for multiplying this cactus in vitro. The researchers compared media containing 6-benzylaminopurine, a cytokinin growth regulator, and combinations of this regulator with the auxin indole-3-acetic acid. After 120 days, the two best treatments produced an average of 5.13 shoots per explant, the plant fragment used to initiate the culture. These treatments were a medium containing 3 mg/L of the cytokinin regulator and a medium containing 1 mg/L of this regulator plus 0.5 mg/L of the auxin. In a separate rooting stage, a medium containing 2 mg/L indole-3-acetic acid yielded 89% of shoots forming roots. When the plants were transferred from culture vessels to substrate, the higher survival rate of 68.75% was obtained in a mixture of peat and local regosol soil at a ratio of 1 to 1. [García-González et al. 2026]

Significance of the study

The paper distinguishes three stages of propagation, shoot formation, root development and acclimatization outside the culture. The resulting protocol provides a basis for multiplying the tested material for collections and conservation outside its natural habitat. [García-González et al. 2026]

Source publication

García-González D.A., Flores-Margez J.P., Osuna-Ávila P., Propagación in vitro y adaptación de plantas a condiciones ex vitro de Kroenleinia grusonii (Cactaceae), Revista Mexicana de Biodiversidad, 2026, 97:e975700, doi: 10.22201/ib.20078706e.2026.97.5700.

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Biology and physiology

Chromosomes and DNA organization in Armatocereus from the Peruvian Andes

A 2026 paper characterized the chromosomes of Armatocereus matucanensis from the Cosma population in the Peruvian region of Áncash, identified in the publication as Armatocereus mataranus subsp. ancashensis. The researchers combined microscopic chromosome imaging with fluorescent labelling of DNA regions encoding ribosomal RNA. Most of the metaphase spreads examined contained 22 chromosomes, corresponding to two sets of 11. The 5S rDNA regions occurred on two chromosome pairs, while 45S rDNA occurred on a different pair. Flow cytometry of five young plants estimated the diploid nuclear DNA content, designated 2C, at 2.73 pg. Stem tissue also contained nuclei with multiples of this DNA content, indicating endopolyploidy. [Rojas et al. 2026]

Significance of the study

The findings provide a reference for comparisons of genome organization among cactus lineages and further studies of material from Áncash. This characterization concerns the population examined and retains its identification from the source paper. [Rojas et al. 2026]

Source publication

Rojas L.G., Garcia-Paitan M., Ciprian-Salcedo G., Siles M., López A., Comparative cytogenetics and rDNA organization in Cactaceae: insights from Armatocereus mataranus subsp. ancashensis, Plant Systematics and Evolution, 2026, 312:48, doi: 10.1007/s00606-026-02028-6.

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Cacti in the wild

Armatocereus laetus roots respond rapidly to small rainfall events

Roots of Armatocereus laetus in a seasonally dry Ecuadorian forest rapidly resumed water uptake following small rainfall events. The paper, published in June 2026, presents 17 months of measurements of this cactus and three co-occurring tree species. Miniature heat-based sensors recorded water flow through roots measuring 2 to 6 mm in diameter. In the cactus, isolated dry-season rainfall events caused rapid increases in flow, whereas tree root activity depended more strongly on leaf presence and soil moisture. During the monitored periods, cactus roots often took up water for almost the entire day and night. Their activity was less closely associated with atmospheric evaporative demand than that of roots of the comparison tree Eriotheca ruizii. [Würzberg et al. 2026]

Significance of the study

The measurements show how root water uptake complements water storage in a succulent stem in an environment with irregular rainfall. The most complete time series came from one root per species, so the findings describe the responses of the monitored roots. [Würzberg et al. 2026]

Source publication

Würzberg L., Homeier J., Coners H., Mundry R., Gonzaga Poma J.A., Windhorst D., Trachte K., Scholz S., Bendix J., Leuschner C., Contrasting diurnal and seasonal root water uptake patterns among different plant life forms in a neotropical dry forest, Trees, 2026, 40:83, doi: 10.1007/s00468-026-02784-5.

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Cacti in the wild

Night-time pollination of Leptocereus leonii by a Cuban bat

Leptocereus leonii is a cactus endemic to the Sierra de Anafe in western Cuba. A 2026 publication describes a study of 243 flowers, covering their structure, opening period, nectar secretion and pollination. The flowers remained functional for less than a day, and nectar production peaked at the beginning of the night. The experiment on the reproductive system and timing of pollination compared 185 flowers on 73 plants. Treatments using self-pollen and pollen from other individuals demonstrated self-incompatibility, with effective pollination occurring at night. The only recorded floral visitor was the bat Monophyllus redmani, identified by the authors as the pollinator. [Ruiz and Barrios 2026]

Significance of the study

In the studied population, fruit formation depended on pollen transfer between plants during nocturnal flower opening. Understanding this relationship allows conservation planning for the site to consider both the cactus and its interaction with the pollinator. [Ruiz and Barrios 2026]

Source publication

Ruiz A., Barrios D., Reproductive biology of Leptocereus leonii Britton & Rose, an endemic cactus from seasonally dry tropical forest of western Cuba, Bradleya, 2026, 44:5–17, doi: 10.25223/brad.n44.2026.a3.

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Biology and physiology

Cells and genes shaping Disocactus flower size

Rosas-Reinhold and colleagues compared flower development in Disocactus speciosus and D. eichlamii. The study was published online on 5 March 2026. The authors sampled buds of both species at two developmental stages and combined microscopy with RNA analysis. Tepal cells were similar in size in early buds. Later growth in D. speciosus involved prolonged cell proliferation and expansion. These differences were associated with a larger final perianth size. [Rosas-Reinhold et al. 2026]

Significance of the study

Transcriptomes identified homologues of BIG BROTHER, BIG PETALp and NAC100 as candidates associated with growth regulation. Their roles require further functional experiments. [Rosas-Reinhold et al. 2026]

Source publication

Rosas-Reinhold I., Ramírez-Castro C.G., Cervantes C.R., Rodríguez-Alonso G., Piñeyro-Nelson A., Rosas U., Arias S., Insights into the genetic bases of differential organ size in cactus flowers: a case study in two contrasting species, Annals of Botany, 2026, 138(4):1021–1034, doi: 10.1093/aob/mcag046.

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Species atlas

Two new Acanthocereus species from western Mexico

Field research in western Mexico led to the description of two new species, Acanthocereus crypticus and Acanthocereus veronicae. The paper appeared in Botany Letters on 17 May 2026. The authors combined comparisons of three chloroplast DNA regions with juvenile and adult stem, flower and fruit characters. Acanthocereus crypticus encompasses populations from Jalisco that had been associated with Acanthocereus rosei for more than 45 years. Its adult stems are slender, cylindrical and slightly mottled, whereas juvenile stems have three or four ribs. Acanthocereus veronicae from Jalisco and Nayarit retains three ribs in both juvenile and adult stems. [González-Zamora et al. 2026]

Significance of the study

Both species have nocturnal flowers, and their distinctness is supported by morphological and chloroplast evidence. The descriptions also document small known populations for which the authors proposed threat categories. [González-Zamora et al. 2026]

Source publication

González-Zamora P., Ruiz-Sánchez E., Carrillo-Reyes P., Sánchez D., Phylogenetic and morphological evidence reveal two new species of Acanthocereus (Cactaceae) from western Mexico, Botany Letters, 2026, online 17 May 2026, doi: 10.1080/23818107.2026.2660211.

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Cultivation and propagation

Pitahaya in a Mediterranean climate. From supports to pollination

In March 2026, Trindade and colleagues published a review of pitahaya cultural practices on the Iberian Peninsula. They combined literature from major production regions with experience of cultivation under Mediterranean conditions. Propagation, support structures, pruning, soil management and pollination were examined. Stem arrangement affects canopy illumination and access to flowers and fruit. Hand pollination was related to cultivar selection and provision of an adequate load of compatible pollen. Bibliometric analysis identified water management and irrigation strategies as important topics for further regional research. [Trindade et al. 2026]

Significance of the study

The review organizes evidence from many sources and proposes multi-year trials comparing production systems. For readers in Poland, it provides a reference for plant biology and planning cultivation under cover. [Trindade et al. 2026]

Source publication

Trindade, A.R., Matias, P., Lacerda, V., Pestana, M., Marques, N., Duarte, A., Pitaya as a New Alternative Crop for Iberian Peninsula: Cultural Practices, Plants, 2026, 15(5):807, doi: 10.3390/plants15050807.

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Biotechnology and applications

Yellow pitahaya after harvest. Chilling and fruit quality

In March 2026, Erazo-Lara and colleagues published a storage study of yellow pitahaya Selenicereus megalanthus ‘Palora’. Ecuadorian fruit was immersed after harvest in 0.1 mM methyl jasmonate or methyl salicylate solutions, while controls received the vehicle solution alone. Following twelve days of transport at 10°C, subsequent storage at 2°C and 10°C was compared. Measurements covered firmness, respiration, soluble solids, acidity and selected fruit compounds. Observation of the 2°C treatment ended after 40 days including transport because of chilling injury, shrivelling and decay. The 10°C treatment was assessed until day 55 including transport. [Erazo-Lara et al. 2026]

Significance of the study

At the end of storage at 10°C, methyl-salicylate-treated fruit contained more measured phenolic compounds than controls. The results concern this cultivar and postharvest procedure, relating storage temperature to quality retention and tissue responses to chilling. [Erazo-Lara et al. 2026]

Source publication

Erazo-Lara, A., Oñate-Bastidas, B.A., García-Pastor, M.E., Padilla-González, P.A., Agulló, V., Serrano, M., Valero, D., Effects of Postharvest Application of Methyl Jasmonate (MeJA) and Methyl Salicylate (MeSA) on Storage of Yellow Pitahaya at Two Temperatures, Horticulturae, 2026, 12(4):398, doi: 10.3390/horticulturae12040398.

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