CACTIBOTANY AND CULTIVATION
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Cacti / Tables and diagrams

Tables and diagrams

Micropropagation stages

Cactus and succulent atlasIndex of former namesTables and diagramsCultivation proceduresRootstocks
05 / IN VITRO • the complete micropropagation cycle
  1. 01InitiationExplant and culture without visible contamination
  2. 02MultiplicationShoots, subculturing and tissue quality control
  3. 03StabilisationLimiting disorders and preparing shoots
  4. 04RootingConnecting the shoot to a functional root system
  5. 05AcclimatisationTransition to lower humidity and independent assimilation

Contamination, material identity and survival are checked at every stage. A large number of shoots in a vessel does not determine the success of the whole cycle. [Bouzroud et al. 2022] [Cortés-Olmos et al. 2025]

An original diagram based on Konrad Szychowski’s material from 2010 and the review by Bouzroud et al. from 2022. Stages may partly overlap depending on the material and protocol. [Bouzroud et al. 2022] [Cortés-Olmos et al. 2025]

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