Hudson S. A., Ecroyd H., Kee T. W., Carver J. A., The thioflavin T fluorescence assay for amyloid fibril detection can be biased by the presence of exogenous compounds, The FEBS Journal, 2009, 276(20):5960-5972, doi: 10.1111/j.1742-4658.2009.07307.x
The authors test the effects of curcumin, quercetin and resveratrol on thioflavin T fluorescence in amyloid-fibril systems. Comparing fluorescence readings with electron microscopy reveals potential interference by coloured and fluorescent compounds. The paper explains the importance of analytical controls and complementary methods for assessing protein aggregation.