Kroenleinia grusonii from new shoots in vitro to plants in substrate

The shoot tips of young Kroenleinia grusonii seedlings were used to develop a method for multiplying this cactus in vitro. The researchers compared media containing 6-benzylaminopurine, a cytokinin growth regulator, and combinations of this regulator with the auxin indole-3-acetic acid. After 120 days, the two best treatments produced an average of 5.13 shoots per explant, the plant fragment used to initiate the culture. These treatments were a medium containing 3 mg/L of the cytokinin regulator and a medium containing 1 mg/L of this regulator plus 0.5 mg/L of the auxin. In a separate rooting stage, a medium containing 2 mg/L indole-3-acetic acid yielded 89% of shoots forming roots. When the plants were transferred from culture vessels to substrate, the higher survival rate of 68.75% was obtained in a mixture of peat and local regosol soil at a ratio of 1 to 1.